FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5704137
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2974885.215302507636125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT6550.066.204839
GCGGATT16100.055.848989
CGCGAGT11850.054.3531959
CGGGATT24500.052.3181089
GCGCATT13400.051.3973859
CGCGTTA3650.050.6672367
GACGATT8100.049.599539
GGCGTAT11100.049.408038
GGGGATT74250.049.213049
GGCGATT22050.047.7196669
GCGTATT16700.047.3507929
GCGGAAT21700.046.726099
GCGCAAT13650.045.7841849
CGCGAAT9450.045.2131779
ACGGATT10150.044.6081669
GGCGACT22000.044.349719
GCGTAAT10050.043.7829559
CGCTAGT7900.043.5901839
GTCGCGA6350.043.183397
GCGGAGT26350.043.0785839
Produced by FastQC (version 0.11.5)