FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8293909
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT32450.069.324139
CGCGATT12600.069.014369
GGGGATT100100.060.432159
GGCGATT34800.059.210599
GACGATT12700.055.5706189
CGTTATT20450.054.5394679
ACGGATT17000.054.48789
CGGTATT30350.054.3968169
CGGGATT33700.053.290029
GGGTATT64250.052.470069
CGCGTTA4500.051.8110857
GGCGGAT29300.051.4000788
CGCGATA9200.049.3146069
GCGCAAT21650.049.188069
GCGCATT27300.048.7024359
CGGCATT27000.048.3100479
CGATCGA4850.048.0721447
GGCGAAT30050.048.0199749
GCGGTAT16500.047.355598
GCGGGAT25800.046.8937578
Produced by FastQC (version 0.11.5)