FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7196393
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT12600.068.838669
GCGGATT31050.067.899269
GGCGATT27800.061.034089
GCGTAAT16950.058.6424879
CGCGTTA4800.058.035527
CGGGATT34450.056.4198079
GGGGATT80350.054.0531049
ACGGATT14550.053.956179
GCGTATT24200.051.8002979
GGGTATT50200.050.9518669
CGTGATT21550.050.2378739
GACGATT12500.050.1426859
GCGGGAT28750.049.988548
CGGTATT24450.049.9759259
CGCGATA8250.049.8817639
GGCGGAT28100.046.864018
GGCGAAT27400.046.443829
GGCGTAT15650.046.1182638
GTCGCGA11350.045.7404487
CGTGCGA11550.044.9484067
Produced by FastQC (version 0.11.5)