FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7756421
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA146270.18857924292660236No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG135840.17513231940349808No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA117430.15139714566808582No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG92900.11977173492774566No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG84370.10877439478852424No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC82370.10619588596338439No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC81780.10543522585996816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT8300.0114.159317
GTTAGCG2950.0109.460111
CGCCGTA4850.089.723387
GCTAGCC28050.083.586031
CGTCGAC8850.076.9284448
TCGTGTT26550.070.856414
CGCGATA5700.067.724147
CCGAGTT9750.063.348539
CTTTCGT29600.062.608351
CGAACGT2550.060.5623251
CGAGTTA5600.060.1601457
GTTCGCG3450.059.004483
GTACCCG15950.058.9733475
CGATCGT1903.274181E-1157.75328145
ACGCGTA2750.057.636612145
TTACGCG3050.057.5379836
TTCGTGT32800.057.140433
CGCACTA5200.056.697771
TTTCGTG38450.055.133642
CCGACGT8550.055.0073055
Produced by FastQC (version 0.11.5)