FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9616031
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA162930.16943580984711884No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG134720.14009938195914717No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA124910.12989766775918257No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG119630.12440683687479792No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC102180.10626005677394344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT10750.093.780947
GTTCGCG7450.085.7889253
CGCGTTC8400.082.36655
CTTTCGT38350.078.8824841
CCGCGTT9300.073.687034
TCGTGTT41550.073.267534
TTCGTGT42150.071.751483
CGTTATA4800.068.637339
GCTAGCC28000.066.61341
AGCGTGA22450.065.1594246
CGTCGAC10400.063.9911048
GTTAGCG2700.063.4662481
CTAGTCG23900.063.408243
CGGCGAT7400.063.2195552
TTACGCG6250.062.2031826
TAAACCG8850.061.052327
TTTCGTG54550.060.998822
TAGTCGA25600.059.4583174
CGCCGTA8100.058.570527
AGTCGAA27000.056.6193475
Produced by FastQC (version 0.11.5)