FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005724994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8599414
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA166310.1933968989049719No Hit
CGCAATGACCCCGCACGATTTCATTGAACACTTCCTCTCCAAAATGCCAG159370.18532658155544088No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG151110.1757212758915898No Hit
CTCTCCACCCAAACCCTTGTAGGATGGCAGCTGTGACCCGGGATTTCGGT148020.17212800779215887No Hit
AACAAGTTGCAGGGAAGTCTTAAGAGAGCCGCCCGAAGCCCCGCACCTCC142790.1660461980316333No Hit
GGCACTAGGTGTCTCCCCCTGTAAGCCCCGGCAAGGCTGCCTGGGACATC125110.14548665757922574No Hit
GCCGGATGGAGTTGTCGGTGTAGATGCACAGCTTCTCGGCCGTCAGGGGG122720.14270739843435842No Hit
GCACACATTTGAAGTAGGACACCGAGGGCGCGCAGGTCTCCTCCGCCTTC116230.13516037255561833No Hit
GCGTGTTTGCGGATGATCTGTTTGTTCTCCTCCGCCTCTGGCATTTTGGA115290.13406727481663286No Hit
TTGGCTTAGTCTTGCTCTTATAAAGGCTTCCGGGTCATGGCACCTGGGAA113120.13154384705748556No Hit
TTTCCCTTCTGGTATCAAAATGCTCCGGAGAGGAGGGACTGTCAGGGGAG106630.12399682117874543No Hit
CCTCCCCTGTTCCCATTACTCTAACTTTCGCATGCACACACACGTACCTT105910.12315955482547997No Hit
GAGGAAGCGTGTGAGGCGGTAGTAGGACAGAAAGTTGTTGGGGCTCCTCA102200.11884530736629263No Hit
CCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAG99560.11577533073765257No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG97770.11369379355383982No Hit
CCTCCCCTACCTAGACCCTCCTAACTTTTGGGGTTTTTTTCCTTTCCTCA96600.11233323572978345No Hit
GTGCTTGTCCATTTTGCTTCCGTTGTCTCTTCTAGACTGCTGGAGTTAAT94700.1101237828531107No Hit
CCTCTGGACCCTCAATGTATCCCTTTGGCATTTCCCCTCAGGTCCGTTAT94150.10948420438881068No Hit
AGGTGAAGCCAAACCTGCTCACCTGCTGCTGGCCCCATGGCCTCTTCTCC94090.10941443219270523No Hit
CCACTTGCATGTTCGTGGCCTCTAAGATGAAGGAGACCATCCCCCTGACG93540.10877485372840523No Hit
TATTACGTATTTTGGCTTTGAAATGGAACTGAAAACTTACCTTCGGCTGG92460.10751895419850702No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC90100.10477458115169243No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC90060.1047280663542888No Hit
CCTGGCTCTTCCCACTAAACTCATTCCTCCTGCTGCCTCTGCCCTCTTAC89220.1037512556088124No Hit
CCCAAAGGGCAGTAAGATAGGAAACAGTGTCTCACGCATACGGTTTGGTT88280.10265815786982693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)