FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8411982
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA159480.1895867109558722No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG127650.15174782827638006No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA116910.13898032592081153No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG86660.10301971639977356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT8800.0110.974847
GTTAGCG2350.089.068251
ACGCGTA5850.084.94419145
CGCCGTA3900.082.275857
GCTAGCC24100.081.060731
CGTTATA3750.066.966589
CGTCGAC5900.066.209898
CGATCGT2650.065.63141145
CGCACTA5500.060.8902971
GTACCCG16150.060.481135
CGGCGAT4400.060.253872
GTTCGCG3550.058.9585653
TACCCGT17300.056.4617166
GTATGCG5350.056.0762565
CGGTATA9750.055.8164376
CGAACGT4250.055.8161051
AGGTTCG6250.053.582825
CCGCGTT4050.051.6803444
CTAGTCG15700.051.103683
GCGATTA3700.050.9136436
Produced by FastQC (version 0.11.5)