FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005724999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005724999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7649072
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT31000.070.325319
CGCGATT9850.068.200029
GGCGATT29950.063.6919489
GGGGATT97000.061.0878379
CGGGATT38400.059.9087569
CGGTATT28450.057.471479
GACGATT12500.057.2905969
ACGGATT16750.056.7533269
GGGTATT59250.054.8711289
GCGTATT25950.053.2395069
GCGGGAT29000.052.885668
CGCGTTA3900.052.000317
GGCGAAT28600.051.6303149
GCGCATT23500.051.5086759
GCGTAAT16550.051.312329
GGCGGAT27250.050.9328738
GCGGATA15400.050.205819
GCGGTAT18750.049.6862958
CGTTATT20400.049.394879
GCGTTAT10950.049.19558
Produced by FastQC (version 0.11.5)