FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7310946
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA303160.4146658995976718No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA164630.22518289698761282No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCAG108230.14803829764301363No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG96680.13224006852191222No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG95680.1308722564767952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGCC42550.0109.1667941
ACCCGTT8350.0106.994767
GTTAGCG2550.093.620491
TCGTGTT37800.084.616054
CTTTCGT39450.080.571591
TTCGTGT42400.075.43243
CGTGTTC44200.072.210155
TTTCGTG48200.071.308832
GTACCCG14000.063.8142935
CGGCGAT4150.062.4446682
CGCCGTA3450.061.2802127
CGGTATA10100.060.7709276
GCGGTCT6050.058.618889
TAGCCCT79200.058.3788383
CTAGTCG13950.057.1955953
CGCACTA4500.056.0831261
CGCGATA5600.056.020567
CCCGGTA10850.055.941234
TCCGTTG9550.054.9853742
CGGGTTA10950.054.1847234
Produced by FastQC (version 0.11.5)