FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7310946
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5125037.010077765586013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT26650.073.9507149
CGCGATT7950.069.218189
GGGGATT85250.065.3751459
ACGGATT15850.062.9773259
CGGGATT27350.062.6998259
GGGTATT55800.061.578489
CGGTATT22650.058.76039
TCGCGAG6100.058.7417768
CGTTATT17150.058.5766339
GGCGATT23100.057.0616239
GACGATT11650.053.8256159
GCGTATT23400.052.775259
GTCGCGA8250.052.7404487
GGCGGAT24850.050.9832658
GCGCATT16800.049.513429
CGCGTTA3950.048.59747
CGCGAAT8150.047.8917929
GCGTAAT13900.047.8748479
GCGGGAT19950.047.4687358
GGCGTAT15950.047.338038
Produced by FastQC (version 0.11.5)