FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6873571
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC316920.4610703810290168No Hit
AACAAGTTGCAGGGAAGTCTTAAGAGAGCCGCCCGAAGCCCCGCACCTCC137190.1995905767176916No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG137090.19944509193256313No Hit
CGCAATGACCCCGCACGATTTCATTGAACACTTCCTCTCCAAAATGCCAG119070.17322873365242028No Hit
TTTCCCTTCTGGTATCAAAATGCTCCGGAGAGGAGGGACTGTCAGGGGAG114850.16708927572000057No Hit
GCACACATTTGAAGTAGGACACCGAGGGCGCGCAGGTCTCCTCCGCCTTC108080.1572399557668059No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG107860.15691988923952338No Hit
CTCTCCACCCAAACCCTTGTAGGATGGCAGCTGTGACCCGGGATTTCGGT103780.15098411000628348No Hit
GCCGGATGGAGTTGTCGGTGTAGATGCACAGCTTCTCGGCCGTCAGGGGG98120.14274967116801443No Hit
GCACGTAGAGCTCCGGGTGTCGGGAAAGCTGGGGGAGTACTGGTCCAGGG97530.1418913109357567No Hit
GATGTGGAGCCTTGTCACCAACCTCTAACTGCAGAACTGGGATGTGGAGC97030.14116388701011454No Hit
TGCAGGCGGCTCTTTTTCACGGGCTCCAGCGACAGGAAGCGGTCCAGGTA94200.13704666759098No Hit
CCAACCTGAAACATTGACGGAGAAGTAGGTGGTGTCACTGCCCGAGGGGC94030.13679934345626166No Hit
AGGTGAAGCCAAACCTGCTCACCTGCTGCTGGCCCCATGGCCTCTTCTCC93470.13598462865954247No Hit
TTGGCTTAGTCTTGCTCTTATAAAGGCTTCCGGGTCATGGCACCTGGGAA92530.13461707167933523No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC90730.13199834554702353No Hit
CAGGAGACGTCCCTGACCTTACAGTCCTGCCGCAGGTACATGATGATGCG90610.1318237638048694No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC89330.1299615585552255No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA88560.1288413257097366No Hit
GGCACTAGGTGTCTCCCCCTGTAAGCCCCGGCAAGGCTGCCTGGGACATC86640.12604801783527078No Hit
CCCTGGGTAGCAACGTGGAGTTCATGTGTAAGGTGTACAGTGACCCGCAG84500.1229346434335224No Hit
GAGGAAGCGTGTGAGGCGGTAGTAGGACAGGAAGTTGTTGGGGCTCCTCA84060.1222945103789573No Hit
GTCCCGCAGCCAGTTGATGCTCTGCACATCGTCCCGCAGCCGACAGCGAA82920.12063598382849323No Hit
GCCCATTTGCAGGTGGTCTTTCGGGGTGCTCCTGTGGGAGATCTTCACTC82790.12044685360782627No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG81180.11810454856725856No Hit
TATTACGTATTTTGGCTTTGAAATGGAACTGAAAACTTACCTTCGGCTGG79890.11622779483910183No Hit
GTGCTTGTCCATTTTGCTTCCGTTGTCTCTTCTAGACTGCTGGAGTTAAT77690.1130271295662764No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG77450.11267796608196816No Hit
CCCCACCCTCTCTCTCTCAGGACTGCCTCCGGGCCTGCCAGGAGCAGATC77400.11260522368940395No Hit
CTTCCTCTCGCCCATCACAGGAGTACCTGGACCTGTCCATGCCCCTGGAC76310.11101943953150407No Hit
CCACTTGCATGTTCGTGGCCTCTAAGATGAAGGAGACCATCCCCCTGACG75310.10956459168021979No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA75180.10937546145955283No Hit
GCGTGTTTGCGGATGATCTGTTTGTTCTCCTCCGCCTCTGGCATTTTGGA74770.10877897384052627No Hit
CCCAAGTAAATGAGTCTCAACGTGTTCTTTAAAGCGGACGCAACAGAGAA74510.10840071339919236No Hit
GCTCCAGATGTTGAAAGGCTGATCTGTTCTCCATGCTGTCTCCTGGCACT74220.10797880752231992No Hit
CCTGGCTCTTCCCACTAAACTCATTCCTCCTGCTGCCTCTGCCCTCTTAC73150.10642212032144571No Hit
CTCCCATTCACCTCGATGTGCTTTAGCCACTGGATGTGCGGCTGCGGGTC72020.10477814224949449No Hit
CCTCTGGACCCTCAATGTATCCCTTTGGCATTTCCCCTCAGGTCCGTTAT71230.10362881244697988No Hit
CAACGCCACCACAAGATGATAAGTCACAGGCTGGAAGACTAGGGGGGCTC71090.1034251337478001No Hit
CCCAAAGGGCAGTAAGATAGGAAACAGTGTCTCACGCATACGGTTTGGTT70780.10297413091390195No Hit
GGCAGGACATCGAGAGGAGAAGTTACAGTGTGTACCTTCCAGAACGGTCA69480.10108282870723238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT12800.0102.398737
CTTTCGT25900.078.5777741
CTAGTCG17600.078.047073
TCGTGTT26750.075.848694
ACGTATT15300.075.781725
GTAATAT60350.073.083241
CTTAGTC21150.072.697175
TAATATA61550.072.171272
GCGTGAT22300.071.4919057
TACCCGT18400.071.23396
TTCGTGT29550.069.3008653
AGTCGAA20400.068.265335
GTACCCG19200.067.9371265
AGCGTGA23750.067.127146
GCTTAGT23000.067.1203464
GCTAGCC18500.067.09521
GTTAGCG1950.064.623781
AGCAACG22600.064.404519
GCACGTA23700.063.8057561
TTTCGTG35650.063.62772
Produced by FastQC (version 0.11.5)