FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6066394
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG148670.24507145431041902No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA98470.1623204823161832No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG91700.15116064007711993No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA90370.14896823384699379No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG81560.1344456031045791No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC81460.1342807605308854No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC80220.13223671261708356No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC75810.12496715511719153No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA69960.11532386455611027No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT65360.10774110616620022No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA62000.102202395690092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT5650.0101.56587
GTTAGCG2250.099.5503161
ACGCGTA3900.087.463066145
CGCCGTA2700.085.5327157
GCTAGCC21050.085.126161
CGATCGT1850.079.02068145
CGTCGAC3100.067.724418
TCGTGTT21850.061.8151784
AGTACCC47300.061.103182
CCCATAA56050.059.0574656
GTTCGCG2300.057.808273
CTAGGAT26250.056.15486145
CGTAGCG1052.4589099E-855.109695140-144
TTTCGTG32650.055.083922
GTACCCG10300.055.0347565
GAGTACC52800.055.016041
TTACGCG2050.054.620466
CTTTCGT24700.054.12671
GTGGTAC36900.053.862299
TACTCGA9250.053.7131733
Produced by FastQC (version 0.11.5)