FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6066394
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4210956.941438356954725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT19850.072.689519
CGCGATT9100.066.8365259
ACGGATT11150.064.413299
GGCGATT23650.058.5477269
CGGGATT25450.058.2203839
GGGGATT68550.055.8780989
GGCGAAT19000.053.4654739
CGGTATT17650.052.422629
GGGTATT43750.052.0585379
CGCGTTA4500.051.7627537
GCGCATT16050.050.3920869
GCGTAAT13000.049.7718779
CGTGATT16950.049.6250579
GCGTATT20050.049.0519879
TGCGAGT17500.048.8048829
GCGGGAT18250.047.862828
GGGCGAT18450.047.694688
GACGATT10100.047.406489
CGCGACT8950.046.2683839
ACGCGAT4350.046.1104978
Produced by FastQC (version 0.11.5)