FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5883980
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG105980.18011617986464942No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA100410.17064979826579968No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA63000.10707038433169386No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA59820.10166587921780834No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG59800.10163188861960781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG2200.0117.092121
ACCCGTT6650.0107.588837
ACGCGTA2800.086.26197145
CGCCGTA3100.076.1624767
GTACCCG9600.073.783655
GCGCGTT1500.073.19197145
CGAACGT2250.069.966161
CGGTTCG1200.063.99258140-144
TTACGCG2100.061.326686
TACCCGT11700.060.5404366
GCTAGCC16850.060.3026351
CGTCGAC3800.060.249758
AGCGTGA14300.060.0401046
CGCACTA4300.059.9075971
TCCCGTA3650.058.80549
TCCGTTG10000.056.5251582
CCCGGTA7500.056.284264
AGGTTCG4300.054.9087645
CGAGTTA7300.054.885047
TTCGCGC2500.054.3763244
Produced by FastQC (version 0.11.5)