FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973792
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA70820.23814711990616694No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG64420.21662577611346054No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA46110.15505455660651452No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG42670.14348683431793482No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG38730.1302377570455499No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC36710.12344508291097696No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC36220.12179735502684788No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA34660.1165515274773757No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT32700.10996061594085935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT4000.0115.285167
CGCCGTA1900.0110.320737
CGTAGCG551.4370016E-10105.450935140-144
GTTAGCG1150.0103.3101041
CGAACGT900.093.1816561
CGATTAG1750.087.8569951
CGCGACT4700.086.21653
TTACGCG1250.083.843756
GCTAGCC10350.081.027531
CGTCGAC2800.074.86058
CGCGCTT3550.074.791469
TTCGTGT12750.073.974813
TCGTGTT13100.072.002014
ACGCGTA1401.10958354E-1071.611275145
CTTTCGT13300.071.462621
CGATCGT1304.964022E-967.47986145
TACCCGT7050.066.401086
CGCGATA2550.063.0198147
GTACCCG7550.062.002615
CCGTATA3450.060.7573669
Produced by FastQC (version 0.11.5)