FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973792
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2078246.98851836308659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT11300.072.125939
GGCGATT12200.068.395779
CGCGAAT4750.065.365269
CGCGTTA1650.062.7242367
CGGGATT13000.062.1965079
ACGGATT6250.062.0969969
GGGGATT41050.060.1934939
GGGTATT23250.057.033539
CGCGATT3300.056.8438389
GCGTATT8550.055.227599
CGTTATT7050.054.1330159
GCGTTAT3350.052.133678
GACGATT5400.051.5079239
GCGTAAT5850.050.8629239
CGGTATT10300.050.2402889
CGACCGA1700.049.464527
GGCGGAT9500.049.023948
TGCGATT7000.048.0512479
TAGCGAG3050.046.6575788
GGCGTAT4800.045.8180968
Produced by FastQC (version 0.11.5)