FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6760019
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4765317.04925533493323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT23300.078.0574659
CGGGATT26050.074.471719
GGGGATT99650.070.891779
CGCGATT9450.066.854239
GGCGATT25400.064.569129
ACGGATT12900.063.8153959
GGGTATT54800.062.4179849
CGCAGAC32250.062.1428383
CGGTATT19300.060.5089049
GCGGGAT21100.059.278768
GCGTATT20850.057.5409859
GACGATT10400.055.2248659
CGTTATT14300.054.4439139
GGCGGAT21500.054.0204778
TGCGATT19350.053.756649
GGCGTAT13200.052.6960538
CGCGAAT9400.052.2743159
CCGCATT9450.051.3224379
CCGCAGA39500.050.5950162
CGTGCGA8700.049.145197
Produced by FastQC (version 0.11.5)