FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6855765
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG112890.16466433724026422No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA101740.14840065258946303No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG70350.10261436907478597No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA69410.10124326023427No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA69010.10065980966383765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT7350.0118.222297
GTTAGCG2650.0100.275571
ACGCGTA3400.085.608734145
TTACGCG2400.077.797676
CGAACGT2650.073.1740651
CGATCGT1950.071.869064145
CGCCGTA3550.068.778377
GCTAGCC18950.065.186641
GTACCCG13700.063.4262855
TCGTGTT26550.063.0225334
CGGCGAT3200.060.5924152
TACCCGT14550.059.720976
CGCACTA5900.057.2117421
CGCGATA4450.056.4819077
CGAGTTA7300.056.0729377
CGGTATA10150.055.8940476
TTCGTGT29950.055.868063
CTTTCGT29200.055.831891
CGATTAG4550.055.2453771
TTTCGTG38550.053.4640662
Produced by FastQC (version 0.11.5)