FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5962999
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT23500.068.443979
GGCGATT21400.060.6609849
CGCGATT10100.060.189299
CGGGATT23900.058.290019
GGGGATT73250.056.1056259
GCGTATT17800.054.786069
ACGGATT10900.052.866959
ACGCGAT4500.052.066528
CGGTATT18400.051.278799
GGGTATT43600.048.94559
GGCGGAT20750.048.828268
GGCGTAT10800.047.493118
CGCTAAT10450.047.265879
CGTTATT16700.047.0191279
CGGTTAT9400.046.482628
CGCGAAT8100.046.1259
CGTGCGA7600.045.8266837
GGCGAAT19500.045.463499
GCGGGAT20550.044.681278
GCGTAAT12200.044.6383329
Produced by FastQC (version 0.11.5)