FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6648932
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4643286.983497500049632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT9700.067.865889
ACGGATT12650.064.794229
GGCGATT23650.061.6737489
GCGGATT22400.061.6579179
CGGGATT27600.058.9270329
GACGATT11700.057.368119
GGGGATT75850.055.4772729
GCGTAAT12400.053.609119
CGTTATT14500.053.4116869
GCGGGAT20000.051.63138
GGGTATT44300.051.5730259
CGGTATT20800.049.9557659
GCGTATT20100.047.2002569
CGACCGA4000.046.7908677
CGCGAAT9000.046.611599
TGCGATT20350.046.3032579
GGCGAAT19800.045.9596829
ACGCGAT4250.045.557038
GCGCATT18300.044.4367759
GGCGTAT11650.044.318718
Produced by FastQC (version 0.11.5)