FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6175191
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3220855.215790086492871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT16750.060.1459
CGCGATT9000.058.432289
CGGGATT24550.051.1011779
CGGTAGT15500.051.097029
GGCGATT24100.050.7408269
GGCGAAT23350.049.385769
GCGCAAT14550.049.2076579
TGCGAGT15150.043.9130829
GGGGATT71550.043.7456179
CGGGAAT25250.043.662159
GCGCATT16300.043.5359159
GGCGAGT32800.043.2704549
GCGGAGT26350.042.560819
CGCTAAT8950.042.4761859
ACGGATT11250.042.2402049
CGCGAGT13550.042.0843359
GGGTATT40650.040.369799
TGCGTTA6450.040.275547
GGCGACT23400.040.074049
CGGTAAT16300.040.037499
Produced by FastQC (version 0.11.5)