FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4063383
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2863377.046763743412816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT5150.071.6954049
GCGGATT13400.071.545229
GGGGATT50050.061.0888029
GGCGATT14300.058.8885659
CGGGATT16650.058.7470939
CGCGTTA1750.055.5235067
GCGGTAT8200.055.29788
GACGATT6000.055.0608069
CGGTATT13000.054.811679
GGCGAAT12750.054.3622329
GGGTATT30950.053.580049
ACGCGAT2600.052.3202258
GCGGGAT12450.051.5097588
GCGTATT11550.051.0367559
GCGCATT9800.049.574569
ACGGATT9150.048.8485459
GGCGTAT7350.048.47298
CGTTATT8750.047.380069
CGGTTAT6850.047.282798
GCGCAAT8900.046.581529
Produced by FastQC (version 0.11.5)