FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7723544
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT27100.055.501559
CGCGTTA4700.054.3461077
CGCGATT12750.048.9678469
ACGGATT15150.046.3618859
CGCGAGT15000.046.3525249
CGGGATT31450.045.7945679
GCGCATT21850.045.4585049
CGCGATA8600.045.3735549
GGGGATT110550.044.8597649
CGGTATT21550.044.4452179
GCGTAAT15000.043.5146149
GCGGATA18850.042.9073839
GGCGGAT31450.041.734778
TAGCGAG10750.040.9194878
GGCGGTA25950.040.7392237
TCGCGAG7300.038.8762258
GCGGTAT17950.038.7354478
GGCGATT37100.038.4379929
CGGTATA17950.038.3394439
CGGTAGT20000.038.3117759
Produced by FastQC (version 0.11.5)