FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12135101
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8505277.00881681990121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT45750.077.9229669
GGGGATT170550.069.676069
CGGGATT51000.067.875369
GGCGATT48550.067.44299
ACGGATT26000.061.6021429
CGCGATT17300.061.59649
GGGTATT97750.060.1892789
GACGATT21000.058.1246039
CGGTATT37700.057.2166759
GCGTATT39500.054.6093379
GCGGGAT42250.054.5706378
CGCGAAT17850.052.8241049
GGCGGAT42350.052.001818
CGTTATT28900.050.504279
GCGTAAT26750.049.7348679
GCGCATT33200.049.604339
GGGCGAT49600.049.0882038
GGCGAAT43450.047.86129
CGCGTTA7900.047.415297
GGCGTAT25100.047.3436368
Produced by FastQC (version 0.11.5)