FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7991669
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG162850.20377470588434032No Hit
ATCTGAGAGTTCTTGTCCTTCTTCACTAAGGCTATAATCTTCTGAGTCGA160040.2002585442415095No Hit
CCCAGATGGCTTTTTACAATGGACTAAACTGAAGAATTACCTGTGCTCTT157940.19763080778245445No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA152360.19064853661982245No Hit
ATTGAAGGAAATAGGGCGATGAATTGATGCTAATGAATGTGTTTTATTAG149290.18680703617729916No Hit
CCTATAGTTCTGGGATAATTTTGGAAGTATAATAGCAGTTCTTTTCTCTT147800.18494259459444579No Hit
AATGTCTTTAAAACGTTTTTATGGTATTTATCCATGATGCTCAAAATTAA139350.17436908360443856No Hit
TCACAAGAAAGTGAAGACTATTCTCAGCCATCAACTTCTAGTAGCATTAT137000.1714285213764484No Hit
GCCCACCACCAAGTTTCTGATCCTTTTTCTTTTCTCTCAGAATCATCGGA132890.16628566573515494No Hit
CTGTAGATTCACTGCTACTGCTTCTTTCACAACATATCTCCCTTATTACA117440.14695303321496422No Hit
TGGCACGCCAAACAAATCTCCTAGAAGATCATTTGAACAATATACAATAT115320.14430027069439438No Hit
TGTATTTTCCACAGATGTTTCATGATTTCCAGTTTTCATCGTGTCTTTTT113110.14153489089700788No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG106890.13375178576590196No Hit
AAGCACTAATTTCATTAGAGAAAGAATATCAAAAAGCTGTGTGAATGCGT106150.132825821489854No Hit
AGACTTTGAACAGTAAGAGTCTTGCCTTACTTAAAATGAGAACATTACCG105990.1326256129977355No Hit
TCACATCTTCTTGGCTGCTATAAATAATGCTACTAGAAGTTGATGGCTGA105790.1323753523825874No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA102570.128346156478703No Hit
CCCCGACTCCAAGCGCGAAAACCCCGGATGGTGAGGAGCAGGTACTGGCC100800.12613135003464232No Hit
TGCTACAATTGAGGTATACGAAATTTAGTTTATCACTTCATAAAATAAAT100670.12596868063479605No Hit
CCCCTTCCATCACATTGCAACAGATGTTGGGCCCTTCGTGAGAATTGGCT100390.12561831577358873No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC94940.118798714010803No Hit
CTTTCTCAGAGATTTCCCCTTTATCTTTCCCTTTATCTTCAGGAAGCCAA91400.11436910112268164No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG88820.11114073918727113No Hit
AAAAGCTGTGTGAATGCGTCAAATAAATATTCATATATACCTGTCTCACT88200.11036493128031204No Hit
CCCAAGACAAAGAAGAGAGTGTGGAATCTAGTTTGCCCCTTAATGCCATT87670.10970174065016956No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC86850.10867567212806237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG2500.0107.651681
ACCCGTT6800.0106.2119457
CGATCGT2400.0102.33927145
ACGCGTA4700.099.53971145
CGCCGTA4850.087.597487
CCCGACT20150.079.433782
TTACGCG3050.074.308926
CGGCGAT3300.068.6764452
CGAACGT3150.067.450931
CGTCGAC6200.067.382198
GCTAGCC23300.066.5679861
CCGACTC24200.066.4331743
GTTCGCG3600.064.921113
GCGGATA4350.064.52919145
TCGTGTT27500.062.325054
CCAAGCG26250.061.5010039
CTTTCGT29250.059.0800931
CGATTAG4200.057.333291
GCGATTA2400.056.070346
CGCGACT7350.055.8875583
Produced by FastQC (version 0.11.5)