FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968666
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA64320.21666297252705422No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG62250.20969014365374883No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA53970.1817988281605273No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG40040.1348753952111824No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA36470.12284979179200353No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG35410.11927916444625297No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT34760.11708962880970779No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC34440.11601170357325478No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG34200.11520325964591505No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC33490.11281161302753492No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC31560.10631037644517774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT3450.0118.621827
GTTAGCG800.0114.7036741
ACGCGTA1900.0100.246124145
TTACGCG850.091.313886
GTTCGCG950.081.7005163
GCTAGCC11850.080.4154361
CGCCGTA2400.079.379487
CGTCGAC2800.075.59958
CGAACGT1000.070.5868761
TCGTGTT12250.067.96874
TAGCGTA852.1645974E-1066.4100954
CTTTCGT13050.065.9892651
GTACCCG6450.063.449955
GCGGATA1701.4551915E-1163.02238145
TACCCGT6850.059.744856
ATCGTTA1550.059.179964
CGTGTTC14350.058.022065
GCTTAGA18850.058.0207566
CGCACTA1600.057.3518371
TTTCGTG16800.057.119622
Produced by FastQC (version 0.11.5)