FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4518960
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG91040.20146228335723265No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA85200.18853895586595146No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA69350.15346451395896402No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG59440.1315346893975605No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC56250.12447554304530245No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG51550.11407491989307275No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC47620.10537822861897428No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA47050.10411687644944853No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC46090.10199249384814206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT6250.0116.8747
ACGCGTA2050.0112.4381145
GTTAGCG1800.0104.369111
TTACGCG2150.084.127646
GTTCGCG2100.079.5043643
CGCCGTA2550.076.388247
CGTCGAC3650.074.332778
GCTAGCC14550.072.209561
GCGATTA1750.071.554726
CGCACTA2800.069.579411
TCGCGCT2300.069.5670855
GTACCCG10700.068.266775
CGAACGT2100.066.26611
CGCGATA3050.066.1464847
TCGTGTT16400.064.9014
TTCGCGC2500.064.001724
CTTTCGT17050.062.8459131
CCCATAA33500.056.8996476
CGATCGT1602.1589585E-856.023846145
TACCCGT13150.055.5478636
Produced by FastQC (version 0.11.5)