FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7313880
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT32650.076.600849
CGCGATT10700.072.784849
GGGGATT104800.065.4919059
GGCGATT30350.063.0039639
CGGGATT39150.062.9125759
GCGGGAT29250.058.446378
ACGGATT17400.057.1307689
CGCGATA5900.054.7315569
GGCGAAT27450.053.5137339
GCGGTAT14000.053.36718
GACGATT13050.052.885389
ACGCGAT5400.052.7640848
GGCGGAT30000.052.7640848
CGGTATT24000.052.7640849
GGGTATT58850.050.9978079
GGGGCGA64550.050.1238337
CGCGAAT11600.047.487679
GCGTATT23050.047.247329
GGGCGAT30450.047.201768
GGGGGAT108150.046.6021778
Produced by FastQC (version 0.11.5)