FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005725046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922375
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACTCCGTCCAGTATTGATCGGGAGAGCCGGAGCGAGCTCTTCGGGGAG133600.4571624107104667No Hit
TGATCAATAATCACCCTGTTGTTTGTTTCAGTGACTCCTTCACACATACT126750.43372257153856025No Hit
CCAACTAAAAAGGCAAATGTAATCTACCAGGCTTTGGCTGTGGTCAACTT124220.4250652294794473No Hit
GAAAGAGTCTGGATTTTTAGGGCTCATACTATCCTCCGTGGTCATGCTCC112270.3841738312160486No Hit
AGAGTAAAGTAGATGATGGAAATATACAGCTTGCAAGGACTCTGGGCTCC110870.37938320715171736No Hit
CCTAAAATTCAATTTGTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGA110720.3788699260019676No Hit
TCAGGGATACATTGTTTTTATAATTTTTCACCACATGATTTTTCTTCTCT107350.36733820950425594No Hit
ATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACT105940.36251336669660805No Hit
CTACGGGCCATTCTAATAGCCTCAAAATCTCTGCACCAGGGGGATGAAAG104990.35926258608152617No Hit
GATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTCT104210.35659352410282735No Hit
CCTGGAGCCTTATTTTTGATCAACGCAAGGGGATTAAAGAAATAACCTCC104160.3564224303862441No Hit
GCTTGAGAAAGTTGGAAACGTTGCCTTAGAAGCCTGTTTTTTCTCCTTTT103800.3551905556268446No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT103520.35423243081397837No Hit
TGAGTACGTATTTTGAAACTCAAGATCGCATTCATGCGTCTTCACCTGGA101370.34687540100089825No Hit
CGCATGTGAGGATCCTGGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCC100890.345232901321699No Hit
AGGAAAGATCATAATTCCTCTGCACATAGGTAATTTCCAAATTCCCAAGG100180.3428033705462167No Hit
CCTGACCGGAGTAACCTTCCCTCATTTCCTCCTGCAGCTGCAAAGCTGTC99250.33962102741776806No Hit
TGCAAGAGAGGAAATGTTCTGTTCTCCTTCACTTTCCACTCACCCGTAGG99220.33951837118781814No Hit
GCCTCTCACATATTGAAATGTACTTGTCCATCTTTCTCCAGGCCAGGAAA97670.3342144659737371No Hit
GGGATTGTGATTGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCA97570.3338722785405706No Hit
CCTTTCATGCTCTCTTCCCCAGGTAATTATGTGGTGACAGATCACGGCTC94460.3232302493690919No Hit
CCATCATCACTGTTCGGCTTCTGTGAAGGCCCTTCCCATGAAATGGACGT93960.32151931220325936No Hit
GAGGTGATTTGTGTTCCTGCAATAATGTCTCAGGGGTGGGCTGACGGGTT90670.3102613456520809No Hit
TCTCTCTTATCTCTGCAGTGTGTAACGGAATAGGTATTGGTGAATTTAAA90390.3093032208392147No Hit
CGGGACAAGGATGCCTGACCAGTTAGAGGGCCCACAGAGGAGGACTCACG88940.30434150305830016No Hit
GGCAAGGCCCTTCGCACTTCTTACACTTGCGGACGCCGTCTTCCTCCATC88350.3023225972026177No Hit
TTCTCCACTTAGATTTTCTCCAATTTTATTTGTGTAGGAAAATCAAAGTC86910.2973950981650199No Hit
GCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCCA86530.29609478591898714No Hit
ATAGGAGCTGGAGGCAGAGATAGTGTGTATGCGACACTTACAGCTGCCCA86190.294931348646221No Hit
CAACTCCATAAACTAAACAGAAAGCGGTGACTTACTGCAGCTGTTTTCAC85990.29424697377988795No Hit
GTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACT85480.2925018178707387No Hit
GTGACAGAACACAGTGACATGAGATGCTCATTAGAGGGACTCTTCCCAAT84520.28921681851234016No Hit
TCTCTCTCTAAAACACTGATTTCCCATTTATATTCATGTGATAATTCAGC83500.28572650669404165No Hit
GTACTGGGAGCCAATATTGTCTTTGTGTTCCCGGACATAGTCCAGGAGGC79770.2729629154369306No Hit
TGAGTTCCTCAAAAGAGAAATCACGCATTTATGTTTTCTCTTCTTAGACC77360.2647161982976175No Hit
TAAAAATCTCCAAAATATATGCCAAAGAAGTAGAATGAGAAAAATGTATA75390.2579751058642371No Hit
TGGCGCTGAGTGTACTTACCTCACTTGCCCAGCGTGTCCTCTCTCCTCCA75340.25780401214765386No Hit
CTTTCCAAGATCATTCTACAAGATGTCAGTGCACTGAAACATGCAGGGGC74710.2556482313187048No Hit
CTGCACATTCAGAGATTCTTTCTGCATCATAATACAATTATGTATCTAAC74510.2549638564523718No Hit
ACAAATAAAGGACCCATTAGAACCAACTCCATAAACAAAACAGAAAGCGG74140.2536977629496557No Hit
ATGGTGCTTTGCTGATTACTTCACCTCTGATTTCTTTCCACTTTCAGAAT73740.25232901321698964No Hit
CCTCCACCTCATTCCAGGCCTAAGATCCCGTCCATCGCCACTGGGATGGT72820.24918088883185766No Hit
AATTCCATATGTCAAATGAATAGTGTCAGGACTTTATTTGAAGCAAAGGG72150.246888233029642No Hit
GACTGTTTGGGAGTTGATGACCTTTGGATCCAAGCCATATGACGGAATCC72070.2466144830831088No Hit
CATTTTGAAAGAGAAAAGAAAGAGACATGCATGAACATTTTTCTCCACCT71860.24589588947345908No Hit
TCAGTTTCTCATCATTTCACTGAGATATGCATCTATTACTTTTACATTTC71810.24572479575687584No Hit
CCCTCACTCTTCAGCTCACAGGGAACCTTTGCTCTTTTTCAGTGACCAAA71160.2435005774412935No Hit
CCTTTGGAAAACCTGCAGATCATCAGAGGAAATATGTACTACGAAAATTC71000.24295307754822704No Hit
CCTCAACACAGTGGAGCGAATTCCTTTGGAAAACCTGCAGATCATCAGAG69980.23946276572992856No Hit
CCAACAAATGTGAACGGAATACACGTCTCTCTTATCTCTGCAGTGTGTAA69410.23751229736087942No Hit
GGCAACCCCGAGTATCTCAACACTGTCCAGCCCACCTGTGTCAACAGCAC67230.23005261131784938No Hit
TCCCAATGGAAGCACAGACTGCAATTTGTACCTGAATGACAAGGTAGCGC63260.2164677702211386No Hit
TGGAAGTCCATCGACATGTTGCTGAGAAAGTCACTGCTGACTATGTCCCG62270.2130801146327901No Hit
CTGTGCCAGGGACCTTACCTTATACACCGTGCCGAACGCACCGGAGCCCA61530.2105479276273579No Hit
GCAGAATCCTGTCTATCACAATCAGCCTCTGAACCCCGCGCCCAGCAGAG60070.20555199110312675No Hit
GAAGACAGCTTCTTGCAGCGATACAGCTCAGACCCCACAGGCGCCTTGAC59750.2044569913169939No Hit
CCAGCTGCTCAGGAGTCATGCTTAGGATGGATCCCTTCTCTTCTGCCGTC58830.20130886693186192No Hit
CTACCCCTCCAGAAGGTTGCACTTGTCCACGCATTCCCTGCCTCGGCTGA56790.194328243295265No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG54740.1873134009153514No Hit
GGAACCTTTGCTCTTTTTCAGTGACCAAAATCATCTGTGCCCAGCAGTGC47360.16205996834766243No Hit
TGCCTTTTTAAACTATCATCCTGTAATCAAAGTAATGATGGCAGCGTGTC45920.15713246931006458No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA42600.1457718465289362No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA36650.12541169425552848No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG34220.1170965396295821No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC31440.10758372898755292No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC30540.10450404208905428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)