FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6951386
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATTTGGTCTGCGGCCTGTCGATTATACACATCTCCGAGCCCAC170020.2445843174296464No Hit
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG102660.14768277865737856No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA101550.14608597479696855No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA88810.1277586944531637No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG87190.12542822395418698No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG73940.10636727697181542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTA2450.0120.832504145
ACCCGTT9500.092.229837
CGCGTTC6000.086.0914155
GTTCGCG5500.084.404193
CTTTCGT27700.082.8464361
CGCCGTA5100.078.207777
TTACGCG5400.077.4949346
CGATCGT3150.077.39598145
GCTAGCC20300.074.7202761
TAGTCGA20100.074.4916844
TCGTGTT31350.072.7874154
CTAGTCG21400.070.577553
AGTCGAA21900.068.6701055
GCGTGAT23850.067.717177
AGCGTGA23900.067.575026
CCGCGTT7650.066.6655654
TTCGTGT34650.066.0439763
CGGACCG21050.062.740181
TCGCGCT7400.062.7351075
CGTCGAC9050.062.1328668
Produced by FastQC (version 0.11.5)