FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6951386
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3682625.2976773265072605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT22000.066.058559
GGGGATT99450.059.072229
GGGTATT46100.056.370429
GGCGATT27500.055.3100439
CGGTATT15500.055.2233629
GCGTAAT12100.053.9461179
CGGGATT28650.052.6600389
GACGATT11850.052.4859129
CGCAGAC39300.051.0835723
ACGGATT14100.048.914629
GGCGAAT27800.048.2893569
GGGGAAT106500.046.199469
GGCGGAT24050.046.0889858
CGCGATT10050.045.9552279
CGCGAGT12200.045.932639
CGGTAGT15250.045.2259679
TGCGATT15250.044.014569
GGGGACT85000.042.961119
CGACCGA3900.042.632317
CGTTATT11000.042.546189
Produced by FastQC (version 0.11.5)