FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6644223
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT24450.066.440969
CGCGATT10000.056.4761169
ACGGATT14250.055.218129
CGGGATT27050.053.955469
GGCGATT25700.053.579849
GGGGATT73250.052.7575349
CGGTATT18900.052.0408869
GCGTATT20500.051.074599
GGGTATT46000.048.833779
TCGCCGA5100.048.525787
GCGTAAT13800.047.362329
CGTTATT15650.046.6292349
GGCGAAT23150.045.502159
TCGCGAG5600.045.3262758
ACGCGAT4300.044.272188
GCGGGAT24200.044.0526478
GCGGTAT13450.043.8771868
CGATATT16700.043.697469
GGCGGAT24550.043.6830838
GTCGCGA8100.043.087947
Produced by FastQC (version 0.11.5)