FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5904864
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT21950.056.9389889
GGCGATT20000.052.6570059
CGGTATT16550.050.983749
CGCGATT9300.050.480879
CGGGATT23600.048.1192789
GGGTATT40800.047.7371259
GGGGATT63200.047.18019
ACGGATT11000.046.716519
GCGTATT18750.046.693449
CGCGATA5050.043.9698267
CGCGTTA3650.043.453547
GCGGGAT20350.042.7104578
CGTTATT15850.042.4282079
GACGATT9050.042.0611089
CGCGTAA5650.041.5462048
TTCGCGA4900.041.4316257
GGCGGAT21200.041.2972648
TCGCGAG4800.040.9730728
GTCGCGA6850.040.751187
CGATATT14700.040.5684629
Produced by FastQC (version 0.11.5)