FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8321856
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4412425.3022066231379155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT29350.064.678559
CGCGATT12300.060.130369
CGGTATT18900.059.0247889
CGGGATT33550.058.235049
GGGGATT109750.057.9552549
GGCGATT32600.054.071219
CGCGAGT16650.053.304759
GCGTAAT14550.050.408259
GGGTATT60100.049.532519
GCGGTAT13850.048.5058828
GACGATT13250.047.446259
CGCGAAT13050.046.7565359
CGCGTTA4750.046.7117927
GGCGTAT15200.045.414248
GCGCATT19850.045.332539
CGGGAAT35050.045.192129
GCGTTAA8000.044.684378
GTACGGA11950.043.839817
GGCGGAT31400.043.5753638
ACGGATT19450.042.7791149
Produced by FastQC (version 0.11.5)