FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7361832
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT25300.062.1246539
CGCGATT11300.061.1337249
ACGGATT15300.058.4064069
CGGGATT35800.056.6499759
GACGATT11000.053.006359
GGGGATT82350.052.487229
ACGCGAT5350.052.1232768
GGCGATT26650.051.1297959
GCGTATT23250.050.701739
CGGTATT24850.048.967479
GCGGGAT26300.047.9545828
GGCGAAT25950.047.1369
GGGTATT52650.046.1034289
CGTTATT21450.045.206099
GCGCAAT15400.042.8001639
GCGCATT21950.041.8664679
CGATATT19550.041.81929
GCGGTAT15200.041.6955038
GGTGCGA19650.041.6066637
CGCTAAT12450.041.2333349
Produced by FastQC (version 0.11.5)