FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7891890
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT31850.062.287929
ACGGATT15100.056.4859549
GGGGATT96150.054.671089
CGCGATT11600.054.4660229
GGCGATT29050.054.154799
CGGGATT32800.053.5494049
CGCGTTA6250.052.566587
GCGTATT23800.051.5001459
GGGTATT58450.049.7229589
CGGTATT25650.049.01739
TCGCGAG7250.048.801878
CGTTATT21700.048.3316969
GGCGTAT14850.048.0771148
GCGGGAT23500.047.3185278
GACGATT15250.047.2300729
CGTCGAC8050.044.7368478
GCGGATA16950.044.7296189
GGCGAAT23900.044.4114579
GTACGGA9700.043.6404767
GGCGGAT29900.042.8954548
Produced by FastQC (version 0.11.5)