FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5257075
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2714695.163879153331463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT7500.063.7598849
GCGGATT15900.058.546819
GGCGATT20500.053.807129
GCGTAAT9550.052.743789
CGGGATT21200.052.030479
GACGATT8550.049.9638829
CGCGAGT10900.049.1360559
GCGGATA12500.047.947439
ACGTAGT9150.045.9907389
CGGTAGT11900.045.5427749
GCGGAAT20650.045.388399
ACGCGAT4400.044.9217348
CGCGATA4400.043.472659
GGGGATT62650.042.438749
GCGGAGT26200.042.3443539
CGCGAAT9350.042.2792789
GGCGAGT27450.042.274319
CGTTAAT8600.042.259469
CGCTAGT8650.042.0151869
ACGGATT10200.041.881499
Produced by FastQC (version 0.11.5)