FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7286818
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3804575.221167867785362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT22200.062.871379
CGCGATT9750.058.1764459
GACGATT11350.054.4675259
GGCGATT27900.052.082579
ACGGATT12900.048.417039
CGCGAGT12500.047.9269339
CGGGATT31200.047.390929
GCGTAAT13500.047.2093479
CGGTATT19100.045.3802959
GGGGATT88200.044.6561939
GGCGAAT29050.044.0972679
GCGTATT16300.043.400749
GGCGGAT20650.043.2085578
TGCGAGT15950.043.1543779
GCGGAGT33050.042.8098079
CGGGAAT28900.042.5619249
CGTGCGA8850.042.488417
CGCGTTA3900.042.488417
GGCGAGT38650.042.3784839
CGGTAGT17300.042.3656129
Produced by FastQC (version 0.11.5)