FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8240239
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5748796.976484541285756No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT26900.066.004779
CGGGATT31400.063.6645289
CGCGATT11800.059.5378959
GCGTATT30350.057.6600049
CGTTATT19750.055.945079
GGGGATT84050.055.0153249
CGGTATT28000.053.831549
GGCGATT26550.053.4036879
GGCGTAT18600.052.536528
GCGTAAT16300.052.113099
CGATCGA3400.050.7186437
GGGTATT64600.050.5209129
CGTGCGA8100.050.4635477
TGCGATT25300.050.236049
CGTGATT21850.048.814399
GCGCATT20150.048.495259
CGTACGA6850.048.483687
CGCGTTA4800.047.9009447
GCGGGAT22850.047.7961278
GACGATT13000.047.65539
Produced by FastQC (version 0.11.5)