FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6505234
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3382425.199536250348565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT21800.060.5028849
CGCGATT7500.060.3197179
GCGTAAT11450.055.6489229
GGGGATT86250.051.787059
CGGGATT23850.051.5621649
CGCGACT10550.050.7328269
GGCGATT28150.050.702799
GGCGAAT27500.049.35259
CGGGAAT26300.048.4547659
ACGGATT11950.046.3888479
CGCGAGT12000.046.1955579
GCGGATA14000.045.9689
GGCGGAT23450.045.9204448
GGCGACT27200.044.9774259
CGGGACT22100.044.977429
GGGTATT48000.043.9388779
GGGGACT70350.043.1126829
GCGGACT23900.042.923019
CGCGCTA5200.042.8871237
CGCGAAT9100.042.7120749
Produced by FastQC (version 0.11.5)