FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005725078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5180652
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG95980.1852662560619783No Hit
CGCAATGACCCCGCACGATTTCATTGAACACTTCCTCTCCAAAATGCCAG80680.1557332938016296No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA73760.14237590172047843No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA70890.13683605847294894No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG67010.12934665366444223No Hit
AACAAGTTGCAGGGAAGTCTTAAGAGAGCCGCCCGAAGCCCCGCACCTCC65150.12575637197789005No Hit
TTGGCTTAGTCTTGCTCTTATAAAGGCTTCCGGGTCATGGCACCTGGGAA63210.12201166957363668No Hit
TTTCCCTTCTGGTATCAAAATGCTCCGGAGAGGAGGGACTGTCAGTGGAG63030.12166422295880905No Hit
GCCGGATGGAGTTGTCGGTGTAGATGCACAGCTTCTCGGCCGTCAGGGGG60060.11593135381415312No Hit
GCGTGTTTGCGGATGATCTGTTTGTTCTCCTCCGCCTCTGGCATTTTGGA57560.11110570638599157No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC55390.10691704441834733No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC53390.1030565264758181No Hit
GGCACTAGGTGTCTCCCCCTGTAAGCCCCGGCAAGGCTGCCTGGGACATC52950.10220721252846166No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA52890.10209139699018578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)