FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3487396
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG74290.21302427369877122No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA71600.20531078202762174No Hit
CTTTCGTGTTCAAACCACGGAGTTCACAACACAGCAGCACACACAGCCGG64330.18446428223235903No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA60740.17417006844075061No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG52190.14965320829639078No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC46230.13256309292090718No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA44670.12808984124544504No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC43180.12381731240157412No Hit
GCCACTTGATTTCTTTTCCTTTCTCTCTCTACCAATATTAAGCCTTAGTG39790.11409659241451214No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC39720.11389586958292089No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG39500.11326502639791983No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT39140.11223273754973626No Hit
AACCTGCACGTTTTAATGGAGTAACCGCTATATCCAGGCACATTCCATGA35170.10084888552948962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGCG1250.0107.5900341
ACCCGTT3700.0107.110937
TTACGCG2150.0105.331696
CGCCGTA1850.095.6347667
ACGCGTA1600.095.54084145
CGTCGAC2700.089.117438
GTTCGCG2000.088.4570853
GCTAGCC13300.079.830361
TCGTGTT18150.078.761874
CTTTCGT18100.077.8221051
TCGCGCT2150.075.7071465
CGAACGT1700.070.782921
TTCGTGT20750.068.5489043
TTTCGTG23350.066.980242
CGCGTAG2150.065.8323066
TAAACCG3150.065.153087
CTAGTCG7750.063.9173773
CGCGACT2700.062.9028133
TTCGCGC2600.062.6030924
CGTGTTC23100.061.578855
Produced by FastQC (version 0.11.5)