FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3812369
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG96440.252966069129195No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA86140.2259487473536796No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG62730.16454335873573622No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC56190.14738867092875846No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC53940.14148682879333033No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA53390.1400441562713368No Hit
CTGATTGATGTTTAGCTCTAGACTAAGTTGCTTTCAAGTGATAACTGTCT53170.13946708726253937No Hit
GCTCTTTTACTTTTGTGACAATTTGTGCTACAAGTGAAGATCCACAGCAG51320.13461446150674292No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA49770.1305487480356702No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC44240.1160433315872624No Hit
GCAAGTGAGTGGTACAAGAGTGCAGACTCACAGTTTAAATTATTCTCTTA42380.11116447542197515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT4600.0106.3331157
ACGCGTA1450.095.62239145
CGTCGAC3400.091.738388
TTACGCG1100.090.224416
GTTAGCG1450.083.128391
CGCCGTA2150.082.428787
CGATCGT853.5526682E-881.56028145
CGATTAG1700.079.245231
TAGTCCG2450.075.45713145
TCGTGTT14300.073.865044
GCTAGCC10500.068.877811
CGCGATA2500.065.217657
CGAACGT1200.064.994991
CTTTCGT16400.064.8508761
CCGACGT5400.063.0138745
GTACCCG7800.062.710925
TAGACTC24700.062.5657779
TGTCGAG7950.062.495354145
CGGCGAT1750.060.763382
GCTTAGA25650.059.9737326
Produced by FastQC (version 0.11.5)