FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14944569
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10493077.021326610355909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT52950.075.276029
CGCGATT22900.073.227129
ACGGATT33000.063.1280949
CGGGATT63700.060.8511969
GGCGATT51350.060.665369
GGGTATT111600.059.6994329
GGGGATT174850.059.5349469
CGGTATT47950.057.434579
GACGATT24800.055.914049
GCGTATT48150.054.115199
GGCGGAT46950.051.7892768
CGCGAAT24900.050.768099
CGCGTTA10250.050.3384937
GCGCATT39600.050.3265729
GCGTAAT32700.050.097969
CGTTATT36100.049.310119
GCGGTAT30400.048.3721478
GCGGGAT49850.047.6120348
GGCGAAT48400.047.039589
CGGTAGT38550.046.678189
Produced by FastQC (version 0.11.5)