FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10262149
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT38600.062.962819
CGGGATT41500.053.3912059
CGCGATT17050.052.574449
CGTTATT28900.051.767989
GGCGATT39650.050.946879
ACGGATT21900.050.4434289
GGGTATT70550.050.2863359
CGGTATT35000.050.1403589
TCGCGAG8600.049.179838
GACGATT16050.049.1638269
GGGGATT106400.048.5906759
GCGTATT33700.045.1437729
TGCGTTA11300.044.6912237
CGCGTTA7500.044.6092877
CGTGATT31900.043.931199
GGCGAAT31600.042.5503589
GCGTAAT21350.042.2813539
CGTTAAT21800.042.277289
CGTGCGA13300.042.2424437
GCGGGAT29050.041.939378
Produced by FastQC (version 0.11.5)