FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6744000
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACCCATAATACAGTGAGCCCACCTTCCTGGTCCCCAGACATTTCAG143440.21269276393831557No Hit
GCAGTGCTTAGACTCAGTACCACCTGCCCAGAGCAAATCTGATGGACTGA113150.16777876631079477No Hit
CACCTTGGAGCCACTATCAAATGCTGTGAAGAGAAATGTACCCAGATGTA82990.12305753262158957No Hit
GAATGTAAATGCAATTTTATTTACCACTTTGATGCTCCAAATGGCACTGC82240.12194543297746145No Hit
TTGGAGGTGTCTTGAGTGCACTGTGTGTGAGGCCTGTGGGAAGGCAACTG81340.1206109134045077No Hit
GCTAGCCCTGTCATTCTAGGAGTTTATTATCCTTCAGACACAGCTACTTA78850.11691874258600238No Hit
GGACAAAGCAGGAAGATGTGACTGAAATCCTGAAAGGAGCCGGCTCCTGC71790.10645017793594305No Hit
GGAGTCTTCCTGGGTCAGTTGCCTTCCCACAGGCCTCACACACAGTGCAC70050.10387010676156584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT10000.0109.138977
ACGCGTA3050.091.95365145
GTTCGCG4200.087.715783
GTTAGCG2300.081.597011
CGTCGAC7550.075.496668
CGAACGT2400.069.508561
CTAGTCG16150.068.00423
TCGCGCT5750.066.4903955
TACCCGT17000.064.198436
CGATCGT1801.2732926E-1163.740604145
GTACCCG17300.063.0837635
TCGTGTT20150.062.439284
GCTAGCC20200.062.2824251
CGCACTA4250.062.148831
TTACGCG3050.061.5371866
TTCGCGC6000.061.401514
AGTCGAA17900.061.357575
TAGTCGA18200.060.3448334
CGCCGTA3300.056.876127
CGCGATA3150.055.170847
Produced by FastQC (version 0.11.5)