FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6744000
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3505545.198013048635825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT23250.070.197189
CGCGATT11400.065.176429
CGCGAGT12950.058.846559
GACGATT9900.056.4490939
GCGTAAT11350.054.8327149
CGGGATT29850.054.0379569
CGTGCGA6800.053.2323277
GCGTATT18550.053.063679
GGCGATT29600.051.7052769
ACGGATT13000.051.292699
CGCGAAT11250.050.2396979
GGCGGAT23550.049.0783588
GCGGAAT26450.048.979469
GCGGAGT35450.048.547029
GCGCATT16250.048.068589
GGGGATT87300.048.010839
CGGTAGT16250.047.6777769
GCGGATA15100.047.1032189
CGGGAAT28900.046.3654149
TGCGTTA7000.046.26817
Produced by FastQC (version 0.11.5)