FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3770547
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1956785.18964489767665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT11550.065.880639
CGCGATT4800.062.0890669
CGCGAGT7050.050.368319
CGGGATT13350.049.8731169
GGGGATT48550.047.802479
ACGGATT6600.047.07729
GGCGATT14750.046.858999
GCGTAAT7200.045.7961859
GGGTATT27050.045.7115369
ACGTAGT5450.044.212559
GGCGGAT11950.044.0421688
CGCGGTA3600.044.0347947
TGCGAGT8750.043.4812169
GCGGAAT13850.043.0364659
CGCTAGT4300.042.7649549
GCGGTAT6100.042.6199078
GGCGAAT14300.042.5690239
GGCGAGT19900.042.0609789
CGGTAGT8750.042.031849
CGGGAGT22900.041.8119059
Produced by FastQC (version 0.11.5)