FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5562790
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2929725.266637784277314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT16600.060.5067569
CGCGATT8650.059.8842479
GCGTAAT11300.055.903169
CGGGATT23600.053.2667129
GGCGTAT9500.050.536468
TGCGAGT13050.049.3747029
ACGGATT10500.049.333219
CGCGAGT10700.048.411099
GCGTATA7300.046.728929
GGCGATT20600.046.6112989
GCGGAGT23800.045.9181069
TGCGATT14200.045.820919
GCGTATT16300.045.7308439
GCGGGAT18250.044.998228
GGGGATT68000.044.9625859
GGGTATT38000.044.8843549
GCCCGAT3950.044.779148
CGTTATT12650.044.444129
GGCGAAT21150.043.6071139
CGGCGTA7550.043.508217
Produced by FastQC (version 0.11.5)