FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4650011
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3267737.027359720224317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATT14800.071.297219
CGCGATT7150.069.290729
CGGGATT17650.063.4300359
GGGGATT58750.062.972429
GGCGATT16150.061.3533949
CGTTATT10550.057.937749
GCGCATT12900.055.8622939
CGCGTTA3000.055.7625357
GGGTATT31400.054.7106679
GGCGGAT13700.052.6002548
ACGGATT8900.052.0514539
CGGTATT13000.050.4832429
CGATCGA2550.050.463837
GACGATT8250.049.913329
GGCGAAT17050.047.925849
GCGGGAT15750.047.387948
GCCCGAT3600.046.4687778
GCGTATT13750.046.3257989
GCGTTAA4850.045.1053058
CGTTAAT8100.044.4829339
Produced by FastQC (version 0.11.5)